RNA sequencing read depth requirement for optimal transcriptome coverage in Hevea brasiliensis

One of the concerns of assembling de novo transcriptomes is determining the amount of read sequences required to ensure a comprehensive coverage of genes expressed in a particular sample. In this report, we describe the use of Illumina paired-end RNA-Seq (PE RNA-Seq) reads from Hevea…

RNA sequencing read depth requirement for optimal transcriptome coverage in Hevea brasiliensis